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Microsoft exchange smtp 550 error

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Smtp 550 is a relay error..  This pretty much means that something is going wrong when your email servr is trying to connect to the outside world.  What sould be happening is you exchange server collect all of the @youdomain.com mail then connects to your ISP's SMTP server as a relay host to send everything.  I would check the user name/password combo for the ISP server.  Can any in the company send mail? 

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